PGK-DTAG-NTERM-GFP
(Plasmid #185775)

• Purpose: Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
• Depositing Labs: Alessandra Ianari, William Sellers
• Publication: Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4.

Backbone

• Vector backbone: PGK-DTAG-NTERM
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Species: Other
• Insert Size (bp): 730
• Mutation: none
• Promoter: PGK
• Tag / Fusion Protein:
  • V5, dTAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry