Systematic profiling of conditional degron tag technologies for target validation studies.
Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, Ianari A
Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4. PubMed Article
Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, Ianari A
Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185760 | SFFV-DTAG-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185761 | SFFV-SMASH-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185762 | SFFV-HALO-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185763 | SFFV-AID-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185764 | SFFV-IKZF3-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185765 | SFFV-DTAG-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185766 | SFFV-SMASH-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185767 | SFFV-HALO-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185768 | SFFV-AID-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185769 | SFFV-IKZF3-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185770 | PGK-DTAG-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185771 | PGK-SMASH-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185772 | PGK-HALO-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185773 | PGK-AID-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185774 | PGK-IKZF3-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185775 | PGK-DTAG-NTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185776 | PGK-SMASH-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185777 | PGK-HALO-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185778 | PGK-AID-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |
| 185779 | PGK-IKZF3-CTERM-GFP | Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning |