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(Plasmid #185909)


Item Catalog # Description Quantity Price (USD)
Plasmid 185909 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Lentiviral vector (Gen 3)
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 9800
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
    HaloTag fused to mouse Fragile X mental retardation protein
  • Alt name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Ctsa (a.k.a. PPCA, Ppgb)
  • Promoter hSyn (human Synapsin promoter)
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer hSyn promoter primer
  • 3′ sequencing primer Wpre reverse primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p323.hSyn.Halo-mFMRP.Wpre was a gift from Young J Yoon (Addgene plasmid # 185909 ; ; RRID:Addgene_185909)
  • For your References section:

    Presynaptic FMRP and local protein synthesis support structural and functional plasticity of glutamatergic axon terminals. Monday HR, Kharod SC, Yoon YJ, Singer RH, Castillo PE. Neuron. 2022 Aug 17;110(16):2588-2606.e6. doi: 10.1016/j.neuron.2022.05.024. Epub 2022 Jun 20. 10.1016/j.neuron.2022.05.024 PubMed 35728596