pTR-452 tNGFR-P2A-Lck
(Plasmid #186080)

Purpose

Knockin of truncated NGFR to target gene
Depositing Lab

Alexander Marson
Publication

Shy et al bioRxiv 2021.09.02.458799 ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	186080	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
PUC19
(Search Vector Database)
Vector type
CRISPR

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
LCK
Species
H. sapiens (human)
Mutation
WT with tNGFR sequence
Entrez Gene
LCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)

Cloning Information

Cloning method Unknown

Resource Information

Article Citing this Plasmid
- 1 Reference

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.09.02.458799v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pTR-452 tNGFR-P2A-Lck was a gift from Alexander Marson (Addgene plasmid # 186080 ; http://n2t.net/addgene:186080 ; RRID:Addgene_186080)
For your References section:

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing. Shy BR, Vykunta V, Ha A, Roth TL, Talbot A, Nguyen DN, Chen YY, Blaeschke F, Vedova S, Mamedov MR, Chung J, Li H, Wolf J, Martin TG, Ye L, Eyquem J, Esensten JH, Alexander M. bioRxiv 2021.09.02.458799 10.1101/2021.09.02.458799

pTR-452 tNGFR-P2A-Lck (Plasmid #186080)