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High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, Eyquem J, Marson A
Nat Biotechnol. 2022 Aug 25:10.1038/s41587-022-01418-8. doi: 10.1038/s41587-022-01418-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
186054pTR-337 pUC19-tNGFR-P2A-CTLA4 NKnockin of truncated NGFR to target gene
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186055pTR-338 pUC19-tNGFR-P2A-LAG3 NKnockin of truncated NGFR to target gene
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186056pTR-342 pUC19-tNGFR-P2A-CD28 NKnockin of truncated NGFR to target gene
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186057pTR-449 tNGFR-P2A-CCR2Knockin of truncated NGFR to target gene
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186058pTR-373 tNGFR-P2A-IL7RKnockin of truncated NGFR to target gene
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186059pTR-457 tNGFR-P2A-STAT1Knockin of truncated NGFR to target gene
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186060pTR-469 JUNB-tNGFRKnockin of truncated NGFR to target gene
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186061pTR-471 IRF4-tNGFRKnockin of truncated NGFR to target gene
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186062pTR-472 FOXO1-tNGFRKnockin of truncated NGFR to target gene
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186063pTR-473 FOXP1-tNGFRKnockin of truncated NGFR to target gene
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186064pTR-345 pUC19-tNGFR-P2A-IL2RB NKnockin of truncated NGFR to target gene
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186065pTR-346 pUC19-tNGFR-P2A-IL2RA NKnockin of truncated NGFR to target gene
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186066pTR-348 pUC19-tNGFR-P2A-ICOS NKnockin of truncated NGFR to target gene
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186067pTR-340 pUC19-tNGFR-P2A-IL2RG NKnockin of truncated NGFR to target gene
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186068pTR-374 tNGFR-P2A-B2MKnockin of truncated NGFR to target gene
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186069pTR-375 tNGFR-P2A-ITGB7Knockin of truncated NGFR to target gene
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186070pTR-459 tNGFR-P2A-STAT6Knockin of truncated NGFR to target gene
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186071pTR-377 tNGFR-P2A-CD5Knockin of truncated NGFR to target gene
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186072pTR-378 tNGFR-P2A-CCR7Knockin of truncated NGFR to target gene
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186073pTR-379 tNGFR-P2A-CD48Knockin of truncated NGFR to target gene
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186074pTR-341 pUC19-tNGFR-P2A-PDCD1 NKnockin of truncated NGFR to target gene
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186075pTR-460 tNGFR-P2A-STAT4Knockin of truncated NGFR to target gene
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186076pTR-458 tNGFR-P2A-STAT2Knockin of truncated NGFR to target gene
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186077pTR-339 pUC19-tNGFR-P2A-TIM3 NKnockin of truncated NGFR to target gene
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186078pTR-450 tNGFR-P2A-CXCR4Knockin of truncated NGFR to target gene
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186080pTR-452 tNGFR-P2A-LckKnockin of truncated NGFR to target gene
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186081pTR-454 tNGFR-P2A-CARD11Knockin of truncated NGFR to target gene
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186082pTR-455 tNGFR-P2A-WASKnockin of truncated NGFR to target gene
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186083pTR-456 tNGFR-P2A-ITGB1Knockin of truncated NGFR to target gene
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186085pIG-712pUC19-tNGFR-P2A-ITCHKnockin of truncated NGFR to target gene
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186086pIG-747pUC19-tNGFR-P2A-CD7Knockin of truncated NGFR to target gene
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186087pIG-735pUC19-tNGFR-P2A-PIK3CDKnockin of truncated NGFR to target gene
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186088pIG-723pUC19-tNGFR-P2A-EVER1Knockin of truncated NGFR to target gene
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186089pIG-727pUC19-tNGFR-P2A-WASPKnockin of truncated NGFR to target gene
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186090pIG-716pUC19-tNGFR-P2A-CD3GKnockin of truncated NGFR to target gene
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186091pIG-714pUC19-tNGFR-P2A-SAPKnockin of truncated NGFR to target gene
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186092pIG-738pUC19-tNGFR-P2A-Kindlin-3Knockin of truncated NGFR to target gene
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186093pIG-731pUC19-tNGFR-P2A-MAGT1Knockin of truncated NGFR to target gene
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186094pIG-728pUC19-tNGFR-P2A-WIPKnockin of truncated NGFR to target gene
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186095pIG-729pUC19-tNGFR-P2A-MoesinKnockin of truncated NGFR to target gene
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186096pIG-702pUC19-tNGFR-P2A-LRBAKnockin of truncated NGFR to target gene
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186097pIG-708pUC19-tNGFR-P2A-DOCK8Knockin of truncated NGFR to target gene
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186098pIG-719pUC19-tNGFR-P2A-CD3ZKnockin of truncated NGFR to target gene
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186099pIG-742pUC19-tNGFR-P2A-Caspase8Knockin of truncated NGFR to target gene
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186100pIG-718pUC19-tNGFR-P2A-CD3DKnockin of truncated NGFR to target gene
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186101pIG-740pUC19-tNGFR-P2A-BCL10Knockin of truncated NGFR to target gene
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186102pIG-704pUC19-tNGFR-P2A-STAT3Knockin of truncated NGFR to target gene
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186103pIG-705pUC19-tNGFR-P2A-STAT5bKnockin of truncated NGFR to target gene
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186104pIG-736pUC19-tNGFR-P2A-CD40LKnockin of truncated NGFR to target gene
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186105pIG-724pUC19-tNGFR-P2A-EVER2Knockin of truncated NGFR to target gene
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186106pIG-748pUC19-tNGFR-P2A-AP3B1Knockin of truncated NGFR to target gene
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186107pIG-734pUC19-tNGFR-P2A-PIK3R1Knockin of truncated NGFR to target gene
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186108pIG-710pUC19-tNGFR-P2A-IL10R1Knockin of truncated NGFR to target gene
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186109pIG-713pUC19-tNGFR-P2A-CD45Knockin of truncated NGFR to target gene
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186110pIG-730pUC19-tNGFR-P2A-Coronin1AKnockin of truncated NGFR to target gene
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186111pIG-717pUC19-tNGFR-P2A-CD3EKnockin of truncated NGFR to target gene
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186112pIG-703pUC19-tNGFR-P2A-BACH2Knockin of truncated NGFR to target gene
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186113pIG-737pUC19-tNGFR-P2A-CD18Knockin of truncated NGFR to target gene
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186114pIG-701pUC19-tNGFR-P2A-FOXP3Knockin of truncated NGFR to target gene
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186115pIG-745pUC19-tNGFR-P2A-CD4Knockin of truncated NGFR to target gene
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186116pIG-721pUC19-tNGFR-P2A-RhoHKnockin of truncated NGFR to target gene
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186117pIG-709pUC19-tNGFR-P2A-IL10Knockin of truncated NGFR to target gene
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186118pIG-744pUC19-tNGFR-P2A-CD2Knockin of truncated NGFR to target gene
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186119pIG-732pUC19-tNGFR-P2A-STIM1Knockin of truncated NGFR to target gene
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186120pIG-720pUC19-tNGFR-P2A-ZAP70Knockin of truncated NGFR to target gene
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186121pIG-746pUC19-tNGFR-P2A-CD5Knockin of truncated NGFR to target gene
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186122pIG-707pUC19-tNGFR-P2A-TTC37Knockin of truncated NGFR to target gene
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186123pIG-648 CTLA4int1-CTLA4-GFP-SV40CTLA4 gene knockin
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186124pIG-719 CTLA4-GFP R70WCTLA4 gene knockin with mutation
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186125pIG-720 CTLA4-GFP R75WCTLA4 gene knockin with mutation
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186126pIG-721 CTLA4-GFP T124PCTLA4 gene knockin with mutation
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186127pIG-641 JE-TRAC-BCMA(BB)28z-P2ABCMA-CAR DNA production
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186128pTR-177 pUC19-CLTA-N-mCherryknockin of mCherry to CLTA target
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Antibodies from Article