pAAV-TRE-Tight2-AkaLuc
(Plasmid
#186186)
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PurposeAAV vector packaging AkaLuciferase under the TRE promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCW3SL
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAkaLuc luciferase
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Alt namefirefly luciferase mutant protein
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SpeciesSynthetic
- Promoter TRE-Tight2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-TRE-Tight2-AkaLuc was a gift from Vladislav Verkhusha (Addgene plasmid # 186186 ; http://n2t.net/addgene:186186 ; RRID:Addgene_186186) -
For your References section:
Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model. Kasatkina LA, Ma C, Matlashov ME, Vu T, Li M, Kaberniuk AA, Yao J, Verkhusha VV. Nat Commun. 2022 May 19;13(1):2813. doi: 10.1038/s41467-022-30547-6. 10.1038/s41467-022-30547-6 PubMed 35589810
