Skip to main content
Addgene
Open Menu

pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
(Plasmid #186980)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186980 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 186980-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 186980-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    N/A
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cluc, Gluc
  • Species
    Cypridina noctiluca, Gaussia princeps

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 186980 ; http://n2t.net/addgene:186980 ; RRID:Addgene_186980)

  • For your References section:

    Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex. Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H. Cell. 2022 May 25. pii: S0092-8674(22)00581-5. doi: 10.1016/j.cell.2022.05.003. 10.1016/j.cell.2022.05.003 PubMed 35643083
Related items:
Commonly requested with: