pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc
(Plasmid
#186987)
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PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneN/A
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecas7-11-s1006-gggs-d122, Gluc crRNA guide
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gRNA/shRNA sequencecrRNA: gTTGatgtcacggaacTGCAGCCAGCTTTCCGGGCATTGGCTTCCATttttttt
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SpeciesD. ishitomonii
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Mutations1006-gggs-d1221 deletion
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains an 18bp deletion in the backbone immediately downstream of the crRNA sequence compared to the depositor's provided sequence. This is not known to affect plasmid function
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 186987 ; http://n2t.net/addgene:186987 ; RRID:Addgene_186987) -
For your References section:
Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex. Kato K, Zhou W, Okazaki S, Isayama Y, Nishizawa T, Gootenberg JS, Abudayyeh OO, Nishimasu H. Cell. 2022 May 25. pii: S0092-8674(22)00581-5. doi: 10.1016/j.cell.2022.05.003. 10.1016/j.cell.2022.05.003 PubMed 35643083
