pUC19-PolB-Arms1
(Plasmid
#187197)
-
PurposePOLB gene homologous arm fragments to tag the POLB gene.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepUC19
-
Backbone manufacturerGenScript
- Total vector size (bp) 3686
-
Vector typeCloning Vector
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameFragments of the PolB gene
-
Alt namePolB
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1057
-
Entrez GenePOLB
- Promoter LacZa
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTAAAACGACGGCCAGT
- 3′ sequencing primer CAGGAAACAGCTATGAC
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byDesigned by us, and purchased from Genscript.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUC19-PolB-Arms1 was a gift from Robert Sobol (Addgene plasmid # 187197 ; http://n2t.net/addgene:187197 ; RRID:Addgene_187197) -
For your References section:
Ubiquitylation of DNA polymerase beta via atypical K27 chains signals a DNA damage response. Fang Q, Koczor CA, Roos WP, McClellan S, Andrews JF, Sobol RW. Nucleic Acids Res. 2026 Apr 13;54(7):gkag344. doi: 10.1093/nar/gkag344. 10.1093/nar/gkag344 PubMed 41978267