Ubiquitylation of DNA polymerase beta via atypical K27 chains signals a DNA damage response.
Fang Q, Koczor CA, Roos WP, McClellan S, Andrews JF, Sobol RW
Nucleic Acids Res. 2026 Apr 13;54(7):gkag344. doi: 10.1093/nar/gkag344.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187197 | pUC19-PolB-Arms1 | POLB gene homologous arm fragments to tag the POLB gene. |
| 187198 | pX330-PolB-gRNA-14-2-hspCas9 | gRNA-2 to target PolB exon 14. |
| 187199 | pX330-PolB-gRNA-14-3-hspCas9 | gRNA-3 to target PolB exon 14. |
| 187206 | pX330-PolB-gRNA-14-4-hspCas9 | gRNA-4 to target POLB exon 14. |
| 187589 | pGEX4T3-GST-HA-Ub | HA tagged ubiquitin. |
| 187590 | pGEX4T3-GST-HA-Ub-K48 | HA tagged ubiquitin with all the Ks mutated into R except K48. |
| 187591 | pGEX4T3-GST-HA-Ub-KO | HA tagged ubiquitin with all the Ks mutated into R. |
| 187592 | pGEX4T3-GST-HA-Ub-K29 | HA tagged ubiquitin with all the Ks mutated into R except K29. |
| 187593 | pGEX4T3-GST-HA-Ub-K27 | HA tagged ubiquitin with all the Ks mutated into R except K27. |
| 187594 | pGEX4T3-GST-HA-Ub(K63) | HA tagged ubiquitin with all the Ks mutated into R except K63. |
| 187606 | pRK5-HA-Ub(K6R-K27R-K29R) | HA tagged ubiquitin with K6R/K27R/K29R mutation. |
| 187607 | pRK5-HA-Ub(K27R-K29R-K48R) | HA tagged ubiquitin with K27R/K29R/K48R mutation. |
| 187608 | pRK5-HA-Ub(K11R-K27R-K29R) | HA tagged ubiquitin with K11R/K27R/K29R mutation. |