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pRK5-HA-Ub(K27R-K29R-K48R)
(Plasmid #187607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187607 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Addgene
  • Total vector size (bp) 4924
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • Mutation
    K27R/K29R/K48R
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GTCGGTCTCTTCACCAAGGCC
  • 3′ sequencing primer TTGTGAAATTTGTGATGCTATTGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-Ub(K27R-K29R-K48R) was a gift from Robert Sobol (Addgene plasmid # 187607 ; http://n2t.net/addgene:187607 ; RRID:Addgene_187607)

  • For your References section:

    Ubiquitylation of DNA polymerase beta via atypical K27 chains signals a DNA damage response. Fang Q, Koczor CA, Roos WP, McClellan S, Andrews JF, Sobol RW. Nucleic Acids Res. 2026 Apr 13;54(7):gkag344. doi: 10.1093/nar/gkag344. 10.1093/nar/gkag344 PubMed 41978267
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