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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 18730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA-DEST47
- Backbone size w/o insert (bp) 7780
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGLUT9-eGFP/pcDNA-DEST47
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SpeciesH. sapiens (human)
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Insert Size (bp)1871
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Mutationdeleted TAA
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GenBank IDNM_020041
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Entrez GeneSLC2A9 (a.k.a. GLUT9, GLUTX, UAQTL2, URATv1)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site Gateway (not destroyed)
- 3′ cloning site Gateway (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GLUT9-eGFP/pcDNA-DEST47 was a gift from Wolf Frommer (Addgene plasmid # 18730 ; http://n2t.net/addgene:18730 ; RRID:Addgene_18730) -
For your References section:
GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor. Takanaga H, Chaudhuri B, Frommer WB. Biochim Biophys Acta. 2007 Dec 14. ():. 10.1016/j.bbamem.2007.11.015 PubMed 18177733