AAV-Ef1a-CreN-InteinN
(Plasmid
#187614)
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PurposeAAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 5841
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCreN-InteinN
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Speciesbacteriophage P1
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Insert Size (bp)514
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC NGS analysis identified a 22 bp insertion in the 3′ ITR sequence. It is unknown what, if any, impact this may have on plasmid function or viral vector preparation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-Ef1a-CreN-InteinN was a gift from Il Hwan Kim (Addgene plasmid # 187614 ; http://n2t.net/addgene:187614 ; RRID:Addgene_187614) -
For your References section:
Neural circuit pathology driven by Shank3 mutation disrupts social behaviors. Kim S, Kim YE, Song I, Ujihara Y, Kim N, Jiang YH, Yin HH, Lee TH, Kim IH. Cell Rep. 2022 Jun 7;39(10):110906. doi: 10.1016/j.celrep.2022.110906. 10.1016/j.celrep.2022.110906 PubMed 35675770