Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

p3xFLAG-Tmprss6
(Plasmid #18789)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18789 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3xFLAG-CMV-14
  • Backbone size w/o insert (bp) 6265
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type II transmembrane serine protease 6
  • Alt name
    Tmprss6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2436
  • Entrez Gene
    Tmprss6 (a.k.a. 1300008A22Rik)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer N-CMV-30 sequencing primer
  • 3′ sequencing primer C-CMV-24 sequencing primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-Tmprss6 was a gift from Bruce Beutler (Addgene plasmid # 18789 ; http://n2t.net/addgene:18789 ; RRID:Addgene_18789)
  • For your References section:

    The serine protease TMPRSS6 is required to sense iron deficiency. Du X, She E, Gelbart T, Truksa J, Lee P, Xia Y, Khovananth K, Mudd S, Mann N, Moresco EM, Beutler E, Beutler B. Science. 2008 May 23. 320(5879):1088-92. 10.1126/science.1157121 PubMed 18451267