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PB-PEmax NG
(Plasmid #187894)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 187894 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEX-A2
  • Backbone manufacturer
    Eurofins Genomics
  • Backbone size w/o insert (bp) 5120
  • Total vector size (bp) 13175
  • Modifications to backbone
    addition of 3' and 5' piggyBac repeats, addition of pU6-pegRNA(B12B) sequence, addition of CAG promoter, addition of bGH (pA)
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; piggyBac transposon
  • Selectable markers
    Puromycin ; thymidine kinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    It is highly recommened to use NEB Stable at 30°C. Cloning efficiency and stability of plasmid can be compromised when using other strains / higher temperatures!
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
  • Species
    Synthetic
  • Insert Size (bp)
    8055
  • Promoter CAG
  • Tags / Fusion Proteins
    • BPSV40 NLS (N terminal on insert)
    • BPSV40 NLS (C terminal on insert)
    • c-Myc NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-PEmax NG was a gift from Tobias Cantz (Addgene plasmid # 187894 ; http://n2t.net/addgene:187894 ; RRID:Addgene_187894)
  • For your References section:

    A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines. Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin U, Petri S, Cantz T. Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2. 10.1038/s41598-021-01689-2 PubMed 34773059