pET15TV-L-hlPYK WT
(Plasmid #187975)

• Purpose: Expresses the human liver pyruvate kinase
• Depositing Lab: Audrey Lamb
• Publication: McFarlane et al Acta Crystallogr F Struct Biol Commun. 2019 Jun 1;75(Pt 6):461-469. doi: 10.1107/S2053230X19007209. Epub 2019 Jun 10.

Backbone

• Vector backbone: pET15TV-L
• Backbone manufacturer: Cheryl Arrowsmith
• Backbone size w/o insert (bp): 7746
• Total vector size (bp): 9375
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hlPYK WT
• Alt name: human liver pyruvate kinase
• Species: H. sapiens (human)
• Insert Size (bp): 1629
• GenBank ID: P30613.2
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ATG CGT CCG GCG TAG A
• 3′ sequencing primer: TAG TTA TTG CTC AGC GGT GG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was codon optimized and synthesized by Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry