CapChR1_pmCherry-N1
(Plasmid
#188031)
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PurposeExpresses CapChR1-mCherry in mammalian cells
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Depositing Lab
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Depositing OrganizationHumboldt-Universitaet zu Berlin
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 188031-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 188031-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepN1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4012
- Total vector size (bp) 5659
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCapChR1-mCherry
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Alt nameCalcium permeable Channelrhodopsin 1
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SpeciesSynthetic; Chlamydomonas reinhardtii
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Insert Size (bp)1647
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MutationS63D, L132C, T159C and N258E mutations in CrChR2
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-C1-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 188031-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 188031-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CapChR1_pmCherry-N1 was a gift from Peter Hegemann (Addgene plasmid # 188031 ; http://n2t.net/addgene:188031 ; RRID:Addgene_188031) -
For your References section:
Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling. Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, Rost BR, Hegemann P. Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4. 10.1038/s41467-022-35373-4 PubMed 36543773
Map uploaded by the depositor.
