pJRoC30_VP11H
(Plasmid #188063)

• Purpose: PROSS-designed versatile peroxidase 11H
• Depositing Lab: Sarel Fleishman
• Publication: Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-3571. doi: 10.1021/jacs.1c12433. Epub 2022 Feb 18.

Backbone

• Vector backbone: pJRoC30
• Backbone manufacturer: California Institute of Technology
• Backbone size w/o insert (bp): 10461
• Total vector size (bp): 11751
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PROSS-designed versatile peroxidase from Pleurotus ostreatus
• Alt name: VP_11H
• Species: Pleurotus ostreatus
• Insert Size (bp): 1290
• Mutation: 38 PROSS mutations, described in publication
• GenBank ID: ASU87523.1 ASU87523.1
• Tag / Fusion Protein:
  • S. cerevisiae native α-factor prepro-leader signal peptide (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pJRoC30_VP11H.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The designed VP sequence is patented.
The pJRoC30 plasmid was provided to the Fleishman lab by EvoEnzyme S.L. via an MTA agreement.