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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18827 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPML1
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Alt nameMucolipin-1
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SpeciesH. sapiens (human)
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Insert Size (bp)1743
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GenBank IDNM_020533
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Entrez GeneMCOLN1 (a.k.a. LECD, MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
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Tag
/ Fusion Protein
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer V5 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEST from Invitrogen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRPML1-CFP was a gift from Craig Montell (Addgene plasmid # 18827 ; http://n2t.net/addgene:18827 ; RRID:Addgene_18827) -
For your References section:
Lysosomal localization of TRPML3 depends on TRPML2 and the mucolipidosis-associated protein TRPML1. Venkatachalam K, Hofmann T, Montell C. J Biol Chem. 2006 Jun 23. 281(25):17517-27. 10.1074/jbc.M600807200 PubMed 16606612