Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18844||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7497
Vector typeYeast Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)647
Entrez GeneSEC4 (a.k.a. YFL005W, SRO6)
/ Fusion Protein
- Cerulean (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site GATEWAY CLONING (not destroyed)
- 3′ cloning site GATEWAY CLONING (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG416GPD-Cerulean-SEC4 was a gift from Susan Lindquist (Addgene plasmid # 18844 ; http://n2t.net/addgene:18844 ; RRID:Addgene_18844)
For your References section:The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis. Gitler AD, Bevis BJ, Shorter J, Strathearn KE, Hamamichi S, Su LJ, Caldwell KA, Caldwell GA, Rochet JC, McCaffery JM, Barlowe C, Lindquist S. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):145-50. 10.1073/pnas.0710685105 PubMed 18162536