Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

His-FLAG-Tev_ACTB (beta-actin)
(Plasmid #188453)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 188453 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJCX4
  • Backbone size w/o insert (bp) 4672
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACTB
  • Alt name
    beta cytoplasmic actin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1122
  • Mutation
    WT
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV_for
  • 3′ sequencing primer WPRE_rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-FLAG-Tev_ACTB (beta-actin) was a gift from Roberto Dominguez (Addgene plasmid # 188453 ; http://n2t.net/addgene:188453 ; RRID:Addgene_188453)
  • For your References section:

    A solution to the long-standing problem of actin expression and purification. Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, Dominguez R. Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5. 10.1073/pnas.2209150119 PubMed 36197995