A solution to the long-standing problem of actin expression and purification.
Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, Dominguez R
Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5. PubMed Article
Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, Dominguez R
Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188452 | His-FLAG-Tev_ACTA1 (alpha-actin) | Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site. |
| 188453 | His-FLAG-Tev_ACTB (beta-actin) | Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site. |
| 188454 | His-MBP_G4G6 in pRSF | Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags. |
| 188455 | His_Naa80d in pTYB12 | Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag. |