His-MBP_G4G6 in pRSF
(Plasmid #188454)

• Purpose: Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.
• Depositing Lab: Roberto Dominguez
• Publication: Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5.

Backbone

• Vector backbone: pRSF-Duet1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3823
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G4G6
• Alt name: gelsolin segments 4-6
• Species: H. sapiens (human)
• Insert Size (bp): 2253
• Mutation: WT
• Entrez Gene: GSN (a.k.a. ADF, AGEL)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-MBP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7_for
• 3′ sequencing primer: T7_term

Resource Information

• Supplemental Documents:
  • hismbp_g4g6-in-prsf.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry