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Addgene

RoL10X_pET29b
(Plasmid #215427)

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Item Catalog # Description Quantity Price (USD)
Plasmid 215427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RoL10X
  • Alt name
    Lipase from Rhizopus oryzae
  • Species
    Rhizopus oryzae
  • Mutation
    V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F216Y/S267L

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene from lipase described in Chow, J. Y. & Nguyen, G. K. T. Rational design of lipase ROL to increase its thermostability for production of structured tags. Int. J. Mol. Sci. 23, 9515 (2022).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RoL10X_pET29b was a gift from Gregg Beckham (Addgene plasmid # 215427 ; http://n2t.net/addgene:215427 ; RRID:Addgene_215427)

  • For your References section:

    Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization. Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT. Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. 10.1038/s41467-024-45523-5 PubMed 38336849
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