pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown
(Plasmid #188634)

Purpose

HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR
Depositing Lab

Jared Toettcher
Publication

Farahani et al bioRxiv (2022) ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	188634	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pUC19
(Search Vector Database)
Total vector size (bp) 4861
Vector type
Mammalian Expression, CRISPR

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
Unknown

Gene/Insert

Gene/Insert name
EGFR
Alt name
ERBB
Species
H. sapiens (human), Synthetic
Entrez Gene
EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA)
Tag / Fusion Protein
- CD3ε-mNeonGreen (C terminal on insert)

Cloning Information

Cloning method Gibson Cloning
5′ sequencing primer agctggcacgacaggtttcccgactggaaagc
3′ sequencing primer tcgcgcgtttcggtgatgacgg

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- pHR EGFR-CD3ε-FusionRed.gb
- pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown.gb

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
- mNeonGreen Label License
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.08.13.503850 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown was a gift from Jared Toettcher (Addgene plasmid # 188634 ; http://n2t.net/addgene:188634 ; RRID:Addgene_188634)
For your References section:

pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. Farahani PE, Yang X, Mesev E, Fomby K, Bashor C, Nelson CM, Toettcher JE. bioRxiv (2022) 10.1101/2022.08.13.503850

Map uploaded by the depositor.

pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown (Plasmid #188634)