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pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells.
Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor CJ, Nelson CM, Toettcher JE
Elife. 2023 May 22;12:e82863. doi: 10.7554/eLife.82863.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
188626pHR EGFR-CD3ε-FusionRedHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor
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188627pHR iRFP-ZtSH2Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor
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188628pHR ErbB2-CD3ε-FusionRedHuman ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor
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188629pHR Clover-VISH2Fluorescent reporter for SLP76/VISH2 pYtag biosensor
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188630pHR EGFR-SLP76-TagBFPHuman EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor
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188631pHR Grb2-TagBFPHuman Grb2 C-terminally labeled with TagBFP
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188632pHR FGFR1-CD3ε-FusionRedHuman FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor
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188633pX330 EGFR-sgRNAA human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR
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188634pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdownHDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR
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Antibodies from Article