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pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells
Farahani PE, Yang X, Mesev E, Fomby K, Bashor C, Nelson CM, Toettcher JE
bioRxiv (2022)
Article

Plasmids from Article

ID Plasmid Purpose  
188626pHR EGFR-CD3ε-FusionRedHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor
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188627pHR iRFP-ZtSH2Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor
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188628pHR ErbB2-CD3ε-FusionRedHuman ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor
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188630pHR EGFR-SLP76-TagBFPHuman EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor
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188631pHR Grb2-TagBFPHuman Grb2 C-terminally labeled with TagBFP
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188632pHR FGFR1-CD3ε-FusionRedHuman FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor
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188633pX330 EGFR-sgRNAA human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR
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188634pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdownHDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR
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Recombinant Antibodies from Article