pHR EGFR-SLP76-TagBFP
(Plasmid #188630)

Purpose

Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor
Depositing Lab

Jared Toettcher
Publication

Farahani et al bioRxiv (2022) ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	188630	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pHR
(Search Vector Database)
Total vector size (bp) 13659
Vector type
Mammalian Expression, Lentiviral

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
Unknown

Gene/Insert

Gene/Insert name
EGFR
Alt name
ERBB
Species
H. sapiens (human), Synthetic
Insert Size (bp)
4716
Entrez Gene
EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA)
Promoter SFFV
Tag / Fusion Protein
- SLP76-TagBFP (C terminal on insert)

Cloning Information

Cloning method Gibson Cloning
5′ sequencing primer ccgacagactgagtcgcccggg
3′ sequencing primer ccagaggttgattatcgataagc

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- pHR EGFR-SLP76-TagBFP.gb

Terms and Licenses

Academic/Nonprofit Terms
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.08.13.503850 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pHR EGFR-SLP76-TagBFP was a gift from Jared Toettcher (Addgene plasmid # 188630 ; http://n2t.net/addgene:188630 ; RRID:Addgene_188630)
For your References section:

pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. Farahani PE, Yang X, Mesev E, Fomby K, Bashor C, Nelson CM, Toettcher JE. bioRxiv (2022) 10.1101/2022.08.13.503850

Map uploaded by the depositor.

pHR EGFR-SLP76-TagBFP (Plasmid #188630)