pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)
(Plasmid #18865)

• Depositing Lab: Tobias Meyer
• Publication: Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Backbone

• Vector backbone: Gateway destination vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Stromal interaction molecule 1
• Alt name: STIM1
• Species: H. sapiens (human)
• Mutation: 3 mutations (A79K, N80D, D82G) in the EF hand of STIM1. YFP inserted between signal peptide(SP)of STIM1 and residues 24-685 of STIM1. (Lab plasmid WS5182)
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry