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pET3d-E2-25K-C170S
(Plasmid #18892)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 18892 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin conjugating enzyme E2-25K
  • Alt name
    E2-25K
  • Species
    H. sapiens (human); Most likely H. sapiens
  • Mutation
    Cysteine 170 is replaced with a Serine residue.
  • Entrez Gene
    UBE2K (a.k.a. E2-25K, HIP2, HYPG, LIG, UBC1)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cecile Pickart
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3d-E2-25K-C170S was a gift from Cecile Pickart (Addgene plasmid # 18892 ; http://n2t.net/addgene:18892 ; RRID:Addgene_18892)
  • For your References section:

    Ubiquitin chain synthesis. Raasi S, Pickart CM. Methods Mol Biol. 2005 . 301():47-55. 10.1385/1-59259-895-1:047 PubMed 15917625