|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18892||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameUbiquitin conjugating enzyme E2-25K
SpeciesH. sapiens (human); Most likely H. sapiens
MutationCysteine 170 is replaced with a Serine residue.
Entrez GeneUBE2K (a.k.a. E2-25K, HIP2, HYPG, LIG, UBC1)
- Cloning method Restriction Enzyme
- 5′ cloning site N7A (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET3d-E2-25K-C170S was a gift from Cecile Pickart (Addgene plasmid # 18892 ; http://n2t.net/addgene:18892 ; RRID:Addgene_18892)
For your References section:Ubiquitin chain synthesis. Raasi S, Pickart CM. Methods Mol Biol. 2005 . 301():47-55. 10.1385/1-59259-895-1:047 PubMed 15917625