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pBCPB+
(Plasmid #18940)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18940 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBCSK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3190
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    attP, lacZ, attB
  • Insert Size (bp)
    4031

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    attP sequence from the plasmid pIJ8600 (a gift from Mervyn Bibb, John Innes Institute, Norwich, U.K.)
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBCPB+ was a gift from Michele Calos (Addgene plasmid # 18940 ; http://n2t.net/addgene:18940 ; RRID:Addgene_18940)
  • For your References section:

    A phage integrase directs efficient site-specific integration in human cells. Groth AC, Olivares EC, Thyagarajan B, Calos MP. Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000. 10.1073/pnas.090527097 PubMed 10801973