|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18940||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3190
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameattP, lacZ, attB
Insert Size (bp)4031
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBCPB+ was a gift from Michele Calos (Addgene plasmid # 18940 ; http://n2t.net/addgene:18940 ; RRID:Addgene_18940)
For your References section:A phage integrase directs efficient site-specific integration in human cells. Groth AC, Olivares EC, Thyagarajan B, Calos MP. Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000. 10.1073/pnas.090527097 PubMed 10801973