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pDrBB2
(Plasmid #18946)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18946 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHZ-attB
  • Backbone size w/o insert (bp) 4622
  • Vector type
    Insect Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hygromycin gene driven by the copia promoter, phiC31 attB
  • Alt name
    phiC31 bacterial attachment site
  • Species
    Escherichia coli
  • Insert Size (bp)
    3686

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (destroyed during cloning)
  • 3′ cloning site PflMI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hygromycin gene driven by the copia promoter from pMK33 (R. Nusse, Stanford University)
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDrBB2 was a gift from Michele Calos (Addgene plasmid # 18946 ; http://n2t.net/addgene:18946 ; RRID:Addgene_18946)
  • For your References section:

    Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397