pDEST27-GST-hADAM10
(Plasmid
#189553)
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PurposeProtein expression ADAM10 in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDEST27
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameADAM metallopeptidase domain 10
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Alt nameADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
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SpeciesH. sapiens (human)
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Entrez GeneADAM10 (a.k.a. AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains L60S, D96G, S115G, Y316H, and S700G mutations in hADAM10 compared to the NCBI reference sequence NP_001101.1. These mutations were present in the original DNA that was was PCR amplified from human placenta cDNA and are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST27-GST-hADAM10 was a gift from Haian Fu (Addgene plasmid # 189553 ; http://n2t.net/addgene:189553 ; RRID:Addgene_189553)
