E42_gRunx1_gRNA3_dTet_NGFR
(Plasmid
#189803)
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PurposeRetroviral delivery of guide RNA against mouse Runx1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepQCXIN
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegRunx1_gRNA3
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gRNA/shRNA sequenceagcggcgaccgcagcatgg
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SpeciesM. musculus (mouse)
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Insert Size (bp)19
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Entrez GeneRunx1 (a.k.a. AML1, CBF-alpha-2, Cbfa2, Pebp2a2, Pebpa2b)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E42_gRunx1_gRNA3_dTet_NGFR was a gift from Ellen Rothenberg (Addgene plasmid # 189803 ; http://n2t.net/addgene:189803 ; RRID:Addgene_189803) -
For your References section:
Runx1 and Runx3 drive progenitor to T-lineage transcriptome conversion in mouse T cell commitment via dynamic genomic site switching. Shin B, Hosokawa H, Romero-Wolf M, Zhou W, Masuhara K, Tobin VR, Levanon D, Groner Y, Rothenberg EV. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2019655118. doi: 10.1073/pnas.2019655118. 10.1073/pnas.2019655118 PubMed 33479171
