pBA2675
(Plasmid #189997)

• Purpose: Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)
• Depositing Lab: Bungo Akiyoshi
• Publication: Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. doi: 10.12688/wellcomeopenres.17964.1. eCollection 2022.

Backbone

• Vector backbone: pBA310 (Derivative of pDEX777)
• Backbone size w/o insert (bp): 5601
• Total vector size (bp): 6597
• Vector type: Expression vector for Trypanosoma brucei
• Selectable markers: phleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: FBP75
• Alt name: Tb927.5.700
• Species: Trypanosoma brucei
• Insert Size (bp): 600
• Mutation: N-terminal 600 bp of FBP75
• GenBank ID: AAZ11185.1
• Tag / Fusion Protein:
  • NLS (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: VhhGFP4
• Species: Synthetic
• Insert Size (bp): 345

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The insert DNA was gene synthesized by GeneArt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry