nLuc-ΔECD-PTHR1
(Plasmid
#190011)
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PurposeExpresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190011 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 190011-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 190011-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTwist CMV Hygro
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Backbone manufacturerTwist Biosciences
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 8472
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenLuc-ΔECD-PTHR1
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Alt namenLuc-dECD-PTHR1
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Alt namenLuc-delNT-PTHR1
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SpeciesH. sapiens (human)
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Insert Size (bp)1890
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Entrez GenePTH1R (a.k.a. EKNS, PFE, PTHR, PTHR1)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTwist Biosciences
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.07.25.500850v1 for bioRxiv preprint.
DNA (Catalog # 190011-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 190011-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nLuc-ΔECD-PTHR1 was a gift from Sam Gellman (Addgene plasmid # 190011 ; http://n2t.net/addgene:190011 ; RRID:Addgene_190011) -
For your References section:
Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay. Yu Z, Cary BP, Kim TW, Nguyen KD, Gardella TJ, Gellman SH. ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25. 10.1021/acschembio.2c00595 PubMed 36282520
