nLuc-PTHR1
(Plasmid #162577)

• Purpose: Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells
• Depositing Lab: Sam Gellman
• Publication: Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Parathyroid Hormone Receptor 1
• Alt name: PTHR1
• Alt name: PTH1R
• Alt name: Parathyroid hormone 1 receptor
• Species: H. sapiens (human)
• Insert Size (bp): 2295
• GenBank ID: AY449732.1
• Entrez Gene: PTH1R (a.k.a. EKNS, PFE, PTHR, PTHR1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Nanoluc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PTHR1 in pcDNA3.1 was acquired from the cDNA Resource Center.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.07.25.500850 for bioRxiv preprint.