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(Plasmid #162577)


Item Catalog # Description Quantity Price (USD)
Plasmid 162577 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Parathyroid Hormone Receptor 1
  • Alt name
  • Alt name
  • Alt name
    Parathyroid hormone 1 receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    PTH1R (a.k.a. EKNS, PFE, PTHR, PTHR1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Nanoluc (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PTHR1 in pcDNA3.1 was acquired from the cDNA Resource Center.

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nLuc-PTHR1 was a gift from Sam Gellman (Addgene plasmid # 162577 ; ; RRID:Addgene_162577)
  • For your References section:

    Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay. Yu Z, Cary BP, Kim TW, Nguyen KD, Gardella TJ, Gellman SH. ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25. 10.1021/acschembio.2c00595 PubMed 36282520