pPG-GPD-TRPC
(Plasmid
#190113)
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Purpose(Empty Backbone) Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Modifications to backboneInserted Aspergillus parasiticus1.6-kb pyrG and Aspergillus nidulans gpdA_trpC expression cassette containing unique NotI and SmaI cloning sites (from pTR1-GPD-TRPC)
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPG-GPD-TRPC was a gift from Perng-Kuang Chang (Addgene plasmid # 190113 ; http://n2t.net/addgene:190113 ; RRID:Addgene_190113) -
For your References section:
Aspergillus flavus aswA, a gene homolog of Aspergillus nidulans oefC, regulates sclerotial development and biosynthesis of sclerotium-associated secondary metabolites. Chang PK, Scharfenstein LL, Li RW, Arroyo-Manzanares N, De Saeger S, Diana Di Mavungu J. Fungal Genet Biol. 2017 Jul;104:29-37. doi: 10.1016/j.fgb.2017.04.006. Epub 2017 Apr 22. 10.1016/j.fgb.2017.04.006 PubMed 28442441
