pcDNA3.3_SARS2_omicron_BA.2.75
(Plasmid #190674)

• Purpose: expressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus production
• Depositing Lab: David Nemazee
• Publication: Dacon et al Cell Host Microbe. 2022 Nov 4. pii: S1931-3128(22)00523-6. doi: 10.1016/j.chom.2022.10.010.

Backbone

• Vector backbone: pcDNA3.3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Spike
• Alt name: BA.2.75 (omicron variant)
• Species: SARS-CoV-2
• Insert Size (bp): 3800
• Mutation: K147E, W152R, F157L, I210V, G257S, D339H, G446S, N460K, Q493R (on the basis of BA.2), c-terminal 18aa deletion
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: TK_pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RBD fragment synthesized by Burton lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry