PRE-Hsp70BbCas9_1.3
(Plasmid #190797)

• Purpose: aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product
• Depositing Lab: Omar Akbari
• Publication: Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024. doi: 10.1093/g3journal/jkad024.

Backbone

• Vector backbone: AttB with mini-white
• Total vector size (bp): 16870
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Follow the same growth instructions as indicated for Addgene plasmid #122486
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
• Species: D. melanogaster (fly)

Resource Information

• Supplemental Documents:
  • PREHsp70BbCas9_1-3.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor recommends running a restriction digest to verify the correct size of the poly PREs, as indicated in the “Cloning and storage of PRExpress plasmid" document for Addgene plasmid #122486: https://www.addgene.org/122486/.