Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible Hsp70Bb promoter in Drosophila melanogaster.
Warsinger-Pepe N, Chang C, Desroberts CR, Akbari OS
G3 (Bethesda). 2023 Apr 11;13(4):jkad024. doi: 10.1093/g3journal/jkad024. PubMed Article
Warsinger-Pepe N, Chang C, Desroberts CR, Akbari OS
G3 (Bethesda). 2023 Apr 11;13(4):jkad024. doi: 10.1093/g3journal/jkad024. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190795 | PRE-Hsp70BbCas9_1.0 | PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process |
| 190796 | PRE-Hsp70BbCas9_1.2 | gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process. |
| 190797 | PRE-Hsp70BbCas9_1.3 | aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product |