pCfuNca-Plastid
(Plasmid #190818)

• Purpose: Plastid targeting GFP with ble resistant gene for zeocin
• Depositing Lab: Peter Kroth
• Publication: Sprecher et al Algal Research. 2023 May; Vol 72: 103136. doi: 10.1016/j.algal.2023.103136

Backbone

• Vector backbone: pBluescriptII SK+
• Backbone size w/o insert (bp): 2920
• Total vector size (bp): 7485
• Vector type: Bacterial Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Diatom plastid targeted GFP
• Alt name: GFP
• Insert Size (bp): 849
• Promoter: Nitzschia captiva fcp

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTCATACCTTCACATC
• 3′ sequencing primer: TAAAAAGGGGTACT

Gene/Insert 2

• Gene/Insert name: Bleomycin resistance gene
• Alt name: ble
• Insert Size (bp): 375
• Promoter: Cylindrotheca fusiformis fcp

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: GATCCCTTTGTATTCA
• 3′ sequencing primer: TGGAAGCATGAAGTAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Nicole Poulsen and Dr. Nils Kröger created the Cylindrotheca ble gene part of this vector

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry