pBIG1b_10xHisTEVATG5_ATG12
(Plasmid #190861)

• Purpose: Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178
• Depositing Lab: Sascha Martens
• Publication: Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 10.1083/jcb.201912098.

Backbone

• Vector backbone: pBIG1b
• Backbone manufacturer: Jan-Michael Peters
• Backbone size w/o insert (bp): 5915
• Total vector size (bp): 8315
• Vector type: Bacterial Expression, Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ATG5
• Species: H. sapiens (human)
• Insert Size (bp): 894
• GenBank ID: NC_000006.12
• Entrez Gene: ATG5 (a.k.a. APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5)
• Tag / Fusion Protein:
  • 6xHis-TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
• 3′ sequencing primer: TCCTCTAGTACTTCTCGACAAGCTTTTAGTCCGTAGGTTGGGG

Gene/Insert 2

• Gene/Insert name: ATG12
• Species: H. sapiens (human)
• Insert Size (bp): 423
• GenBank ID: NC_000005.10
• Entrez Gene: ATG12 (a.k.a. APG12, APG12L, FBR93, HAPG12)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
• 3′ sequencing primer: CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC

Resource Information

• Supplemental Documents:
  • pBIG1b_10xHisTEVATG5_ATG12.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://hub.asap.science/shared-research/94a2d9aa-2bf9-4325-8a07-513dccf4c4fb