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A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy.
Fracchiolla D, Chang C, Hurley JH, Martens S
J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 10.1083/jcb.201912098. PubMed Article

Plasmids from Article

ID Plasmid Purpose
16907610xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII
16907710xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
1690796xHis-TEV-ATG3 (Human autophagy E2-like enzyme)Human ATG3
190037pCAG-WIPI2d (FRRG/AAAA)-TSExpression of wipi2d mutant
190237pET-Duet1-6xHis-TEV-LC3BGlydelta5CPlasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.
190860ReactionC_pBIG1a_ATG7_ATG10Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179
190861pBIG1b_10xHisTEVATG5_ATG12Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178
190862pGEX-4T1_ATG4B_BamHI_NotIPlasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

Antibodies from Article