A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy.
Fracchiolla D, Chang C, Hurley JH, Martens S
J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 10.1083/jcb.201912098.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169076 | 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme) | Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII |
| 169077 | 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme) | Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII |
| 169079 | 6xHis-TEV-ATG3 (Human autophagy E2-like enzyme) | Human ATG3 |
| 190037 | pCAG-WIPI2d (FRRG/AAAA)-TS | Expression of wipi2d mutant |
| 190237 | pET-Duet1-6xHis-TEV-LC3BGlydelta5C | Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893. |
| 190860 | ReactionC_pBIG1a_ATG7_ATG10 | Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179 |
| 190861 | pBIG1b_10xHisTEVATG5_ATG12 | Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178 |
| 190862 | pGEX-4T1_ATG4B_BamHI_NotI | Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392 |