mADAM10 E385A in pQCXIP cFLAG
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19137||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2300
Entrez GeneAdam10 (a.k.a. 1700031C13Rik, MADM, kuz, kuzb, kuzbanian)
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mADAM10 E385A in pQCXIP cFLAG was a gift from Zena Werb (Addgene plasmid # 19137 ; http://n2t.net/addgene:19137 ; RRID:Addgene_19137)
For your References section:The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10. Lemieux GA, Blumenkron F, Yeung N, Zhou P, Williams J, Grammer AC, Petrovich R, Lipsky PE, Moss ML, Werb Z. J Biol Chem. 2007 May 18. 282(20):14836-44. 10.1074/jbc.M608414200 PubMed 17389606