hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)
(Plasmid
#191389)
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PurposeDonor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus
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Depositing Lab
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Depositing OrganizationUniversite Laval
Located in Canada -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 191389-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 191389-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2364
- Total vector size (bp) 7220
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehCdt1(1-100)
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SpeciesH. sapiens (human)
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Insert Size (bp)999
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Tag
/ Fusion Protein
- mScarlet-I (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
--Expression is driven by the human PGK1 promoter and BGH polyA
-Use in combination with eSpCas9(1.1)_NoFlag_hROSA26_Left (Plasmid #191442)
-hCdt1(1-100) sequence is derived from the Fucci(CA) system PMID: 29107535
DNA (Catalog # 191389-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 191389-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100) was a gift from Yannick Doyon (Addgene plasmid # 191389 ; http://n2t.net/addgene:191389 ; RRID:Addgene_191389)
