pRetroSuper shSmad4J hygro
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19151||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namesmall hairpin RNA against SMAD family member 4
Alt nameDPC4, JIP, MADH4
SpeciesH. sapiens (human)
Insert Size (bp)64
Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
19nt targeting sequence - 5'-GGTGTGCAGTTGGAATGTA- 3' (SMAD4)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRetroSuper shSmad4J hygro was a gift from Joan Massague (Addgene plasmid # 19151 ; http://n2t.net/addgene:19151 ; RRID:Addgene_19151)
For your References section:TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J. Cell. 2008 Apr 4. 133(1):66-77. 10.1016/j.cell.2008.01.046 PubMed 18394990
Map uploaded by the depositor.