pBAD-T-GECO1
(Plasmid
#191616)
-
PurposeBacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 191616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepBAD
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameT-GECO1
-
SpeciesSynthetic
-
Insert Size (bp)1200
-
Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.10.12.562058 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBAD-T-GECO1 was a gift from Robert Campbell (Addgene plasmid # 191616 ; http://n2t.net/addgene:191616 ; RRID:Addgene_191616) -
For your References section:
A blue-shifted genetically encoded Ca (2+) indicator with enhanced two-photon absorption. Aggarwal A, Sunil S, Bendifallah I, Moon M, Drobizhev M, Zarowny L, Zheng J, Wu SY, Lohman AW, Tebo AG, Emiliani V, Podgorski K, Shen Y, Campbell RE. bioRxiv. 2023 Oct 17:2023.10.12.562058. doi: 10.1101/2023.10.12.562058. Preprint. 10.1101/2023.10.12.562058 PubMed 37905143