pDN-LexAOx4-mNeonGreen
(Plasmid
#192604)
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PurposeVector for inducible mNeonGreen expression with 4x LexA operator (LexAO)
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 192604-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 192604-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDN100
- Backbone size w/o insert (bp) 3651
- Total vector size (bp) 4362
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsNone.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen
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Alt namemNG
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SpeciesSynthetic
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Insert Size (bp)711
- Promoter Minimal promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer ctcgagtccgagcggagactc
- 3′ sequencing primer ccacagaagtaaggttccttc
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 192604-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 192604-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDN-LexAOx4-mNeonGreen was a gift from Yingxiao Wang (Addgene plasmid # 192604 ; http://n2t.net/addgene:192604 ; RRID:Addgene_192604) -
For your References section:
Engineering light-controllable CAR T cells for cancer immunotherapy. Huang Z, Wu Y, Allen ME, Pan Y, Kyriakakis P, Lu S, Chang YJ, Wang X, Chien S, Wang Y. Sci Adv. 2020 Feb 19;6(8):eaay9209. doi: 10.1126/sciadv.aay9209. eCollection 2020 Feb. 10.1126/sciadv.aay9209 PubMed 32128416
Map uploaded by the depositor.
