Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Ppst93-ANME1G37 MCR-NG
(Plasmid #192773)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 192773 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pMEV5
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 8400
  • Vector type
    Synthetic Biology ; Heterologous expression in Methanococcus maripaludis
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Methyl coenzyme M reductase
  • Alt name
    MCR
  • Insert Size (bp)
    4000
  • GenBank ID
    GCA_003194425.1
  • Tag / Fusion Protein
    • Flag-Strep2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ppst93-ANME1G37 MCR-NG was a gift from William B. Whitman (Addgene plasmid # 192773 ; http://n2t.net/addgene:192773 ; RRID:Addgene_192773)
  • For your References section:

    Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea. Shao N, Fan Y, Chou CW, Yavari S, Williams RV, Amster IJ, Brown SM, Drake IJ, Duin EC, Whitman WB, Liu Y. Commun Biol. 2022 Oct 20;5(1):1113. doi: 10.1038/s42003-022-04057-6. 10.1038/s42003-022-04057-6 PubMed 36266535