Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19293||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAlan Friedman
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)810
/ Fusion Protein
- mouse ERalpha ligand-binding domain (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE-5 (Common Sequencing Primers)
No epitope tag - can be detected on western blot using alpha-ER Ab
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabePuro-hSLUG.ER.NoTag was a gift from Eric Fearon (Addgene plasmid # 19293 ; http://n2t.net/addgene:19293 ; RRID:Addgene_19293)
For your References section:The SLUG zinc-finger protein represses E-cadherin in breast cancer. Hajra KM, Chen DY, Fearon ER. Cancer Res. 2002 Mar 15. 62(6):1613-8. PubMed 11912130
Map uploaded by the depositor.